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Classroom: Automated 3D cell detection

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Thumbnail Name Size Date Modified Description Tags File Name
Figure3A_DAPI_Fluorescent 6.74 MB 2014-01-10 11:20:58 30 um thick tissue section from the mouse cerebral cortex. Staining via histochemistry with DAPI stain. Imaged with fluorescent microscopy, 63x 1.4 NA objective.
Figure3A_DAPI_Fluorescent_blueUPdate.jpx
Figure3B_Sox2_Fluorescent 1.37 MB 2014-01-10 11:21:23 50 um thick tissue section from the mouse hippocampus. Staining via immunohistochemistry with anti-Sox2 primary antibody. Imaged with fluorescent microscopy, 20x 0.7 NA objective.
Figure3B_Sox2_Fluorescent_red.jpx
Figure3C_NeuN_Fluorescent 3.61 MB 2014-01-10 11:21:59 30 um thick tissue section from the mouse cerebral cortex. Staining via immunohistochemistry with anti-NeuN primary antibody. Imaged with fluorescent microscopy, 60x 1.4 NA objective.
Figure3C_NeuN_Fluorescent_singlechannel.jpx
Figure3D_NeuN_Brightfield 26.00 MB 2014-01-10 11:20:39 30 um thick tissue section from the mouse striatum. Staining via immunohistochemistry with anti-NeuN primary antibody. Imaged with brightfield microscopy, 100x 1.4 NA objective.
Figure3D_NeuN_Brightfield.jpx
Figure3A_DAPI_Fluoresecent_centroid_markers.tif 27.64 MB 2014-02-28 14:48:37 30 um thick tissue section from the mouse cerebral cortex. Staining via histochemistry with DAPI stain. Imaged with fluorescent microscopy, 63x 1.4 NA objective. Red dots indicate the centroids of cells completely contained within the image boundaries. Yellow dots indicate the centroids of cells that touch at least one image boundary. Horizontal and vertical lines indicate the XY position of the midpoint of each centroid.
Figure3A_DAPI_Fluoresecent_centroid_markers.tif
Figure3B_Sox2_Fluorescent_centroid_markers.tif 48.68 MB 2014-02-28 14:48:51 50 um thick tissue section from the mouse hippocampus. Staining via immunohistochemistry with anti-Sox2 primary antibody. Imaged with fluorescent microscopy, 20x 0.7 NA objective. Red dots indicate the centroids of cells completely contained within the image boundaries. Yellow dots indicate the centroids of cells that touch at least one image boundary. Horizontal and vertical lines indicate the XY position of the midpoint of each centroid.
Figure3B_Sox2_Fluorescent_centroid_markers.tif
Figure3C_NeuN_Fluorescent_centroid_markers.tif 20.57 MB 2014-02-28 14:49:03 30 um thick tissue section from the mouse cerebral cortex. Staining via immunohistochemistry with anti-NeuN primary antibody. Imaged with fluorescent microscopy, 60x 1.4 NA objective. Red dots indicate the centroids of cells completely contained within the image boundaries. Yellow dots indicate the centroids of cells that touch at least one image boundary. Horizontal and vertical lines indicate the XY position of the midpoint of each centroid.
Figure3C_NeuN_Fluorescent_centroid_markers.tif
Figure3D_NeuN_Brightfield_centroid_markers.tif 23.81 MB 2014-02-28 14:48:23 30 um thick tissue section from the mouse striatum. Staining via immunohistochemistry with anti-NeuN primary antibody. Imaged with brightfield microscopy, 100x 1.4 NA objective. Red dots indicate the centroids of cells completely contained within the image boundaries. Yellow dots indicate the centroids of cells that touch at least one image boundary. Horizontal and vertical lines indicate the XY position of the midpoint of each centroid.
Figure3D_NeuN_Brightfield_centroid_markers.tif
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