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Classroom: Automated 3D cell detection

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Figure3A_DAPI_Fluorescent 30 um thick tissue section from the mouse cerebral cortex. Staining via histochemistry with DAPI stain. Imaged with fluorescent microscopy, 63x 1.4 NA objective.
Figure3B_Sox2_Fluorescent 50 um thick tissue section from the mouse hippocampus. Staining via immunohistochemistry with anti-Sox2 primary antibody. Imaged with fluorescent microscopy, 20x 0.7 NA objective.
Figure3C_NeuN_Fluorescent 30 um thick tissue section from the mouse cerebral cortex. Staining via immunohistochemistry with anti-NeuN primary antibody. Imaged with fluorescent microscopy, 60x 1.4 NA objective.
Figure3D_NeuN_Brightfield 30 um thick tissue section from the mouse striatum. Staining via immunohistochemistry with anti-NeuN primary antibody. Imaged with brightfield microscopy, 100x 1.4 NA objective.
Figure3A_DAPI_Fluoresecent_centroid_markers.tif 30 um thick tissue section from the mouse cerebral cortex. Staining via histochemistry with DAPI stain. Imaged with fluorescent microscopy, 63x 1.4 NA objective. Red dots indicate the centroids of cells completely contained within the image boundaries. Yellow dots indicate the centroids of cells that touch at least one image boundary. Horizontal and vertical lines indicate the XY position of the midpoint of each centroid.
Figure3B_Sox2_Fluorescent_centroid_markers.tif 50 um thick tissue section from the mouse hippocampus. Staining via immunohistochemistry with anti-Sox2 primary antibody. Imaged with fluorescent microscopy, 20x 0.7 NA objective. Red dots indicate the centroids of cells completely contained within the image boundaries. Yellow dots indicate the centroids of cells that touch at least one image boundary. Horizontal and vertical lines indicate the XY position of the midpoint of each centroid.
Figure3C_NeuN_Fluorescent_centroid_markers.tif 30 um thick tissue section from the mouse cerebral cortex. Staining via immunohistochemistry with anti-NeuN primary antibody. Imaged with fluorescent microscopy, 60x 1.4 NA objective. Red dots indicate the centroids of cells completely contained within the image boundaries. Yellow dots indicate the centroids of cells that touch at least one image boundary. Horizontal and vertical lines indicate the XY position of the midpoint of each centroid.
Figure3D_NeuN_Brightfield_centroid_markers.tif 30 um thick tissue section from the mouse striatum. Staining via immunohistochemistry with anti-NeuN primary antibody. Imaged with brightfield microscopy, 100x 1.4 NA objective. Red dots indicate the centroids of cells completely contained within the image boundaries. Yellow dots indicate the centroids of cells that touch at least one image boundary. Horizontal and vertical lines indicate the XY position of the midpoint of each centroid.
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